Regulatory

Part:BBa_K4767001:Design

Designed by: Fangjun Liu   Group: iGEM23_CUG-China   (2023-10-04)


Pars


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and changes the local structure of the promoter to activate the transcription of the ars genes and clear arsenic in the cell.


Source

This operon was found in E. coli, which contains arsR (transcriptional regulator), arsB (arsenate permease), and arsC (arsenate reductase).

References

Jia X, Bu R, Zhao T, et al. Sensitive and specific whole-cell biosensor for arsenic detection[J]. Applied and Environmental Microbiology, 2019, 85(11): e00694-19.